3024 - Cellular Electron Microscopy Equipment

The Astbury BioStructure Laboratory (ABSL) at the University of Leeds has identified a requirement for four instruments to perform correlative light and electron microscopy (CLEM) and electron microscopy of cells & tissues. These instruments are:

-A high-pressure freezer to allow rapid freezing of large and/or labile specimens whilst minimizing structural deformation; -And a freeze-substitution unit for low-temperature substitution of water by dehydrating agents and fixatives; -A cryo-ultramicrotome, for room-temperature and cryo-sectioning of samples for (cryo-)EM imaging; -And a cryo-CLEM system, to image cryo-fixed samples by fluorescent microscopy, identifying interesting biological events for further analysis at high-resolution by cryo-EM.