Soil Sample Metabarcoding for Natural England's Long Term Monitoring Network (LTMN)

This project aims to analyse soil samples from Natural England's Long Term Monitoring Network using metabarcoding, to generate data which can be used to explore how past tRFLP analysis of microbial communities can be made compatible with modern metabarcoding approaches. This project will specifically deliver metabarcoding of a limited number of samples using two primer sets: those used for tRFLP, and those used in modern metabarcoding, and deliver both raw and taxonomically assigned data, with advice on use of sequence data for in-silico tRFLP for comparison.

Additional information: To apply please respond to the attached Request for Quotation. Responses should be emailed to matthew.j.shepherd@naturalengland.org.uk and victoria.sloan@naturalengland.org.uk quoting reference "LTMNSOIL - METABARCODING" and "Final Submission", no later than 17:00 on 19th December 2022. Clarification questions can be submitted by emailing the above addressed prior to 15th December 2022; noting that unless commercially sensitive, the question and response will be circulated to all tenderers that request this.