# RQ0000010125 Epifluorescence Microscope

> Contract award record from UK public-sector procurement sources.

## Key facts

- Public buyer: [Defence Science and Technology Laboratory](/buyers/defence-science-and-technology-laboratory/contract-awards)
- Contract winner: [CARL ZEISS LTD](/contract-winners/carl-zeiss/public-sector-contract-awards)
- Award value: £172,121
- Tender value: £100,000
- Award date: 5 October 2023
- Published: 23 October 2023
- Contract start: 5 October 2023
- Contract end: 5 October 2029
- Stage: awarded
- Procurement category: goods
- Notice ID: cefdfb5f-cb3e-4062-919e-ca51726538c5-689138
- OCID: ocds-b5fd17-e6585b21-3b0d-4d31-8c28-8f3dc8fa7ec1

## Description

Intended uses \(priority order\): 1\) Real-time imaging of cellular physiology using ion-sensitive fluorophores or similar probes \(e\.g\. fura 2 and fluo dyes with ability for acquisition of fura 2 ratiometric images ≥1Hz\) 2\) General epifluorescence imaging of living cell cultures using a range of fluorescent dyes to image cellular structure and function \(cell viability, mitochondrial function, ROS etc\)\. Excitation/emission capabilities should cover standard range of fluorophores \(e\.g\. DAPI, fluorescein, rhodamine etc\)\. 3\) General transmitted light and epifluorescence imaging of fixed / stained cell cultures and slide-mounted tissue sections Essential requirements: • Research-grade inverted microscope chassis, stage, transmitted light illumination, eyepieces etc • Microscope should be capable of phase and differential interference contrast transmitted light methods\. • Objectives 10, 20, 40 and ~60 or 100x optimised for epifluorescence observation and DIC, 20 and 40x objectives optimised for UV excitation for Fura 2 \(@340 & 380 nm, if different from standard epifluorescence objectives\), additional 20 and 40x for phase contrast\. • Epifluorescence light source to provide illumination for cellular physiology studies \(excitation suitable for a range of dyes including fura 2\) should include software controlled shutter, or equivalent, to control illumination and synchronise to image acquisition\. • Epifluorescence light source for general epifluorescence imaging \(2 and 3 above\) if different • Ability to mount both epifluorescence illumination systems and switch between physiology and high-resolution epifluorescence imaging modes • Monochrome camera suitable for real-time imaging cellular physiology • Monochrome camera for high resolution general epifluorescence \(if different from above\) • Ability to mount both cameras and switch between physiology and high-resolution epifluorescence imaging modes • Image acquisition and analysis software suitable for running cellular physiology studies • Image acquisition and analysis software suitable for general epifluorescence studies\. Ability to combine fluorescence images to produce composite images, ability to undertake image analysis/cytometry measurements of fluorescently labelled specimens\. Desirable requirements: • Motorised control of microscope operation • Additional image analysis options for analysis of standard histopathology tissue sections Other: • If all capability is not possible on one microscope chassis then would consider two systems\. This requirement will be on the defence sourcing portal \(DSP\) Additional information: How to apply - This notice can be found on the defence sourcing portal at this link: https://www\.contracts\.mod\.uk/go/172903630186FEB197D9

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## Source

[View the original notice on Contracts Finder](https://www.contractsfinder.service.gov.uk/Notice/cefdfb5f-cb3e-4062-919e-ca51726538c5-689138)