# RBGKEW1309 Long-Read Ash Genome Sequencing 2024

> Contract award record from UK public-sector procurement sources.

## Key facts

- Public buyer: [Royal Botanic Gardens, Kew](/buyers/royal-botanic-gardens-kew/contract-awards)
- Contract winner: Novogene \(UK\) Company LTD
- Award value: £150,000
- Tender value: £150,000
- Award date: 26 July 2024
- Published: 22 August 2024
- Contract start: 19 August 2024
- Contract end: 18 August 2025
- Stage: awarded
- Procurement category: services
- Notice ID: c1725ab5-9241-4833-b1ea-aa636dc877f6-779501
- OCID: ocds-b5fd17-f0a5cd0e-71a2-47f9-a409-e20abaae72f9

## Description

Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees \(Fraxinus excelsior\) at a minimum of 15x whole genome coverage via a long-read technology\. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp\. In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates\. Therefore, the total number of samples we wish to sequence is 306\. RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml \(as measured with a Quantus flurometer dsDNA assay\); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1\.8; minimum OD260/230 of 1\.8; minimum DIN of 7\.

## Related contract awards

- [RBGKEW1398 - Fungarium Sequencing Project Phase 2](/contracts/ocds-b5fd17-d77de6aa-703c-4280-ac20-029d5ba74b2f): Royal Botanic Gardens, Kew
- [RBGKEW1378 - Market Research Framework](/contracts/ocds-b5fd17-7762a60d-682f-4802-8da0-64f435875ad8): Royal Botanic Gardens, Kew
- [GB-LONDON: RBGKEW1371 - Fungarium Sequencing Project Phase 1](/contracts/ocds-b5fd17-a0c99d0d-2a75-43f9-825f-8fdec2b96082): Royal Botanic Gardens, Kew
- [Laboratory Equipment](/contracts/ocds-b5fd17-4443695c-a0ee-41ac-a6dc-777976bc8b98): NEWCASTLE UNIVERSITY
- [Supply of genomics DNA sample](/contracts/ocds-b5fd17-44958a34-ddbd-49dc-aa98-23c33b22fbb4): NEWCASTLE UNIVERSITY
- [UKRI - RE22104 - Human Whole Genome Sequencing](/contracts/ocds-b5fd17-90ecfe6c-1b7a-4423-9cba-d3ff58eccac5): UK SHARED BUSINESS SERVICES LIMITED

## Source

[View the original notice on Contracts Finder](https://www.contractsfinder.service.gov.uk/Notice/c1725ab5-9241-4833-b1ea-aa636dc877f6-779501)